Asian Journal of Research in Chemistry

P. Nagaraju ¹, B. Sridhar ², P. Jitendra Kumar ³, U. Upendra Rao ¹, K. Hanumantha Rao ², P. N. V. Gopal ¹

Affiliations
1 Hindu College of Pharmacy, Amaravathi Road, Guntur – 522 002, Andhra Pradesh, IN
2 DCRM Pharmacy College, Inkollu, Andhra Pradesh, IN
3 Siddhartha Institute of Pharmacy, Sattenapalli, Andhra Pradesh, IN

Abstract

The present work describes a simple reverse phase HPLC method for the estimation of Atorvastatin calcium in bulk and pharmaceutical formulations. The estimation was carried out on phenomenex C₁₈ (250 × 4.6 mm, 5μ) column using a mobile phase containing of Acetonitrile: Ortho-phosphoric acid (0.1%): Tetrahydrofuran (48:0.04:52). The eluent was monitored at 244 nm. The results have been validated statistically and recovery studies confirmed the accuracy of proposed method.

Keywords

HPLC, Validation, Atorvastatin Calcium and Pharmaceutical Formulation.

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P. Nagaraju ¹, D. Narendra ¹, P. Jitendra Kumar ¹, V. D. N. Srinivas ¹, S. V. N. Padma ¹

Affiliations
1 Hindu College of Pharmacy, Amaravathi Road, Guntur-522002, IN

Abstract

A reverse phase high performance liquid chromatographic method (HPLC) has been developed for the estimation of entacapone in its dosage forms using C₁₈ column. The mobile phase (acetonitrile and 20 mM potassium dihydrogen phosphate, P^H 3.5) was pumped at a flow rate of 1 ml/min in the ratio of 80:20 and the eluents were monitored at 320 nm. The intra- and inter- day variation was found to be less than 2% showing high precision of the assay method. The mean recovery of the drug from the solution containing 200 or 300 μg/ml was 99.66±1.20% indicating high accuracy. The proposed HPLC method may be used for determining entacapone in bulk drug samples or in pharmaceutical dosage forms.

Keywords

Estimation, Entacapone, Tablets and HPLC.

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